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KMID : 0363120180310030174
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Korean Journal of Pain
2018 Volume.31 No. 3 p.174 ~ p.182
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Orexin-A inhibits capsaicin-induced changes in cyclooxygenase-2 and brain-derived neurotrophic factor expression in trigeminal nucleus caudalis of rats
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Kooshki Razieh
Abbasnejad Mehdi
Mahani Saeed Esmaeili
Raoof Maryam
Aghtaei Mohammad Mehdi Moeini
Dabiri Shahriar
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Abstract
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Background: The trigeminal nucleus caudalis (Vc) is a primary central site for trigeminal transmitting. Noxious stimulation of the trigeminal nociceptors alters the central synaptic releases and neural expression of some inflammatory and trophic agents. Orexin-A and the orexin 1 receptor (OX1R) are expressed in pain pathways including trigeminal pain transmission. However, the the mechanism(s) underling orexin-A effects on trigeminal pain modulation have not been fully clarified.
Methods: Trigeminal pain was induced by subcutaneous injection of capsaicin in the upper lip in rats. The effect of trigeminal pain on cyclooxygenase-2 (COX-2) and brain-derived neurotrophic factor (BDNF) expression in the Vc of animals was determined by immunofluorescence. Subsequently, OX1R agonist (orexin-A) and antagonist (SB-334867-A) was administrated in the Vc to investigate the possible roles of the Vc OX1R on changes in COX-2 and BDNF levels following pain induction.
Results: The data indicated an increase in COX-2 and decrease in BDNF immuno-reactivity in the Vc of capsaicin, and capsaicin- pretreated with SB-334867-A (80 nM), groups of rat. However, the effect of capsaicin on COX-2 and BDNF expressions was reversed by a Vc microinjection of orexin-A (100 pM).
Conclusions: Overall, the present data reveals that orexin-A can attenuate capsaicin-induced trigeminal pain through the modulation of pain effects on COX-2 and BDNF expressions in the Vc of rats.
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KEYWORD
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Brain-derived neurotrophic factor (BDRF), Capsaicin, Cyclooxygenase 2 (COX 2), Orexin-A, Orexin receptor antagonists, Orofacial pain, Rats, Pain measurement, Pain perception, Trigeminal caudal nucleus, Trigeminal neuralgia
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